Composite
P+RBS+NrFA
Part:BBa_K1866000:Design
Designed by: Stefan Oehler Group: iGEM15_IIT_Delhi (2015-09-16)
Promoter+RibosomeBindingSite+NrfA (in pSB1A3)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 380
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None. The sequence is compatible with the 3A assembly recommended by iGEM.
Source
The Promoter+RBS part was obtained from the Biobrick BBa_K880005.
The gene was isolated from Pseudomonas aeruginosa genome. (Refer Biobrick BBa_K1153001)